Journal: British Journal of Cancer

Article Title: Novel antitenascin antibody with increased tumour localisation for Pretargeted Antibody-Guided RadioImmunoTherapy (PAGRIT R )

doi: 10.1038/sj.bjc.6600818

Figure Lengend Snippet: Hydroxylapatite chromatography of ST2146 ( A ) and BC4 ( B ). SDS–PAGE analysis under reducing conditions of ST2146 not digested ( C , lane 1) or digested with sialidase ( C , lane 2) and BC4 peaks 1, 2 or 3 from hydroxylapatite chromatography ( D , P1, P2 and P3, respectively). Note : The dotted chain was found to be not functional. Therefore, divalent BC4 corresponds only to peak 3.

Article Snippet: Briefly, hydroxyl apatite chromatography analysis was carried out by loading 100–300 μ g of protein A-purified BC4 or ST2146 onto a 1 ml Macro-Prep Ceramic Hydroxylapatite Type II (20 μ m) column, 10 × 6 mm 2 (BIO-RAD, Milan, Italy) equilibrated at a flow rate of 0.5 ml min −1 with 10 m M sodium phosphate, pH 6.8.

Techniques: Chromatography, SDS Page, Functional Assay

Journal: British Journal of Cancer

Article Title: Novel antitenascin antibody with increased tumour localisation for Pretargeted Antibody-Guided RadioImmunoTherapy (PAGRIT R )

doi: 10.1038/sj.bjc.6600818

Figure Lengend Snippet: ELISA plates coated with tenascin at 5 μ g ml −1 ( A ) or 0.5 μ g ml −1 ( B ). BC4 peaks 1–3 were obtained by subjecting the protein A eluted BC4 to hydroxylapatite chromatography as shown in .

Article Snippet: Briefly, hydroxyl apatite chromatography analysis was carried out by loading 100–300 μ g of protein A-purified BC4 or ST2146 onto a 1 ml Macro-Prep Ceramic Hydroxylapatite Type II (20 μ m) column, 10 × 6 mm 2 (BIO-RAD, Milan, Italy) equilibrated at a flow rate of 0.5 ml min −1 with 10 m M sodium phosphate, pH 6.8.

Techniques: Enzyme-linked Immunosorbent Assay, Chromatography